This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQCXIP-HA.Tetherin
catalog :
41069
citations: 1
Reference
Hammonds J, Wang J, Yi H, Spearman P. Immunoelectron microscopic evidence for Tetherin/BST2 as the physical bridge between HIV-1 virions and the plasma membrane. PLoS Pathog. 2010;6:e1000749 pubmed publisher
product information
Catalog Number :
41069
Product Name :
pQCXIP-HA.Tetherin
article :
doi10.1371/journal.ppat.1000749
id6001
pubmed_id20140192
bacterial resistance :
Ampicillin
cloning :
backbonepQCXIP
backbone_mutation
backbone_originClontech
backbone_size7162
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
This is a viral-delivery-compatible HA.Tetherin expression construct. It was generated by cloning of tetherin from plasmid pCMV-HA.Tetherin (Addgene plasmid #41068) into AgeI and PacI restriction sites of pQCXIP (Clontech).
origin :
37
pi :
alt_names
BST-2
bone marrow stromal cell antigen 2
CD317
cloning
clone_methodRestriction Enzyme
cloning_site_3PacI
cloning_site_5AgeI
promoterCMV/MSV
sequencing_primer_3pCDH-rev (GCATTCCTTTGGCGAGAG)
sequencing_primer_5LNCX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD317, HM1.24, TETHERIN
geneBST2
id684
genbank_ids
NM_004335.2
NP_004326.1
mutation
nameTetherin
shRNA_sequence
size550
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagIRES Puro
locationN terminal on insert
tagHA
resistance markers :
1389
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA