This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT7-Venus-cdc25C5
catalog :
40969
product information
Catalog Number :
40969
Product Name :
pT7-Venus-cdc25C5
article :
doi
id5982
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepT7-Venus-C1 (785)
backbone_mutationFrom pEYFP-C1: EYFP was replaced with Venus using AgeI and BsrGI restriction sites, and the CMV promoter was replaced with a T7 promoter using NdeI and NheI sites. Note that the SacI and HindIII sites in the MCS are no longer unique.
backbone_originJonathan Pines Lab (Gurdon Institute, University of Cambridge, UK)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
In Vitro Transcription
origin :
37
pi :
alt_names
cdc25C
Cell Division Cycle 25 Homolog C
M-phase inducer phosphatase 3
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5KpnI
promoterT7
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC25, PPP1R60
geneCDC25C
id995
genbank_ids
NM_022809.2
NP_073720.1
mutation
namecdc25C5
shRNA_sequence
size1215
species
9606
Homo sapiens
tags
locationN terminal on insert
tagVenus
resistance markers :
1380
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA