This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST/hCx43-EGFP-N1
catalog :
40907
citations: 3
Reference
Lemcke H, Peukert J, Voronina N, Skorska A, Steinhoff G, David R. Applying 3D-FRAP microscopy to analyse gap junction-dependent shuttling of small antisense RNAs between cardiomyocytes. J Mol Cell Cardiol. 2016;98:117-27 pubmed publisher
Shishido S, Nguyen T. Induction of Apoptosis by PQ1, a Gap Junction Enhancer that Upregulates Connexin 43 and Activates the MAPK Signaling Pathway in Mammary Carcinoma Cells. Int J Mol Sci. 2016;17: pubmed publisher
Smyth J, Vogan J, Buch P, Zhang S, Fong T, Hong T, et al. Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane. Circ Res. 2012;110:978-89 pubmed publisher
product information
Catalog Number :
40907
Product Name :
pDEST/hCx43-EGFP-N1
article :
doi10.1161/CIRCRESAHA.111.257964
id5965
pubmed_id22328533
bacterial resistance :
Kanamycin
cloning :
backbonepegfp-N1
backbone_mutationGateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
backbone_originClontech
backbone_size4722
promoterCMV
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
GJA1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP Rev
sequencing_primer_5CMV Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
geneGJA1
id2697
genbank_ids
NM_000165.3
mutationNo stop codon to create c-terminal fusion
nameConnexin 43
shRNA_sequence
size1146
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
877
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA