This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-HA-KLF5-his
catalog :
40904
citations: 2
Reference
Citron F, Segatto I, Musco L, Pellarin I, Rampioni Vinciguerra G, Franchin G, et al. miR-9 modulates and predicts the response to radiotherapy and EGFR inhibition in HNSCC. EMBO Mol Med. 2021;13:e12872 pubmed publisher
Zhao K, Sikriwal D, Dong X, Guo P, Sun X, Dong J. Oestrogen causes degradation of KLF5 by inducing the E3 ubiquitin ligase EFP in ER-positive breast cancer cells. Biochem J. 2011;437:323-33 pubmed publisher
product information
Catalog Number :
40904
Product Name :
pcDNA3-HA-KLF5-his
article :
doi10.1042/BJ20101388
id5963
pubmed_id21542805
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationFor the construction of the KLF5 plasmid pcDNA3-HA-KLF5-His, in which an HA tag was added to the N-terminus and a histidine tag was added to the C-terminus of KLF5, we first made the pcDNA3-HA-His vector by inserting a fragment of DNA containing an HA tag, EcoRI, XhoI, HindIII, BamHI, NotI, PstI and XbaI cloning sites and a histidine tag into the pcDNA3.1 vector (Invitrogen) between restriction enzyme sites NcoI and ApaI. The KLF5 cDNA was then cloned into this pcDNA3-HA-His vector between the HA and histidine tags at EcoRI and NotI sites using a PCR-based approach with the following primers (restriction enzyme sites are underlined): 5 -TTTGAATTCATGGCTACAAGGGTGC-3 (forward) and 5 -TTTGCGGCCGCGTTCTGGTGCCTCTTCATA-3 (reverse).
backbone_originInvitrogen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
KLF5
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5EcoR1
promoterCMV
sequencing_primer_35 -TTTGCGGCCGCGTTCTGGTGCCTCTTCATA-3
sequencing_primer_55 -TTTGAATTCATGGCTACAAGGGTGC-3
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBTEB2, CKLF, IKLF
geneKLF5
id688
genbank_ids
NM_001730.3
mutation
nameKruppel-like factor 5
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
locationC terminal on insert
tagHis
resistance markers :
1381
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA