psiCheck-2-3 x mir-34 | Addgene 40831 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

psiCheck-2-3 x mir-34

catalog :

40831

citations: 1

Reference
Han B, Hung J, Weng Z, Zamore P, Ameres S. The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol. 2011;21:1878-87 pubmed publisher

product information

Catalog Number :

40831

Product Name :

psiCheck-2-3 x mir-34

article :

doi	10.1016/j.cub.2011.09.034
id	5939
pubmed_id	22055293

bacterial resistance :

Ampicillin

cloning :

backbone

psiCheck-2

backbone_mutation

backbone_origin

promega

backbone_size

6273

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Insect Expression

RNAi

growth notes :

AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC the triple(3x)-repeated sequence: 5'TGGCAGTGACCTTAGCATCAACAC-3' 3'ACCGTCACTGGAATCGTAGTTGTG-5' pairs with dme-miR-34 (uggcagugugguuagcugguugug) with seed(red) plus 3' complementary region (blue) fashion.
5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGA
TGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGC
CAGC
The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA S:

growth strain :

Luciferase reporter

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	SV40
sequencing_primer_3
sequencing_primer_5	RLucF
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CR43033, 34, CR33326, CR43033, Dmel CR43033, Dmel_CR33326, Mir-34, dme-miR-34, dme-mir-34, miR-34
gene	mir-34
id	12798087

genbank_ids

mutation

name

three sites partially complementary to miR-34

shRNA_sequence

size

species

7227

Drosophila melanogaster