This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAFMW-Nbr-D435A/E437A
catalog :
40829
citations: 1
Reference
Han B, Hung J, Weng Z, Zamore P, Ameres S. The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs bound to Drosophila Argonaute1. Curr Biol. 2011;21:1878-87 pubmed publisher
product information
Catalog Number :
40829
Product Name :
pAFMW-Nbr-D435A/E437A
article :
doi10.1016/j.cub.2011.09.034
id5939
pubmed_id22055293
bacterial resistance :
Ampicillin
cloning :
backbonepAFMW
backbone_mutation
backbone_originMurhpy Lab, Carnegie Institution for Science
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expresses Flag, Myc tagged mutant Nbr in Drosophila cells
origin :
37
pi :
alt_names
Nibbler
Nbr
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterActin5C
sequencing_primer_3SV40pA-R
sequencing_primer_5AC5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG9247, CG9247, Dmel CG9247, nbr
geneNbr
id35385
genbank_ids
mutationD435A/E437A, part of the DEDD motif; mutations predicted to block exonuclease activity
nameNbr D435A/E437A
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tag3xFLAG
locationN terminal on backbone
tag6xMyc
resistance markers :
1193
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA