This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-alphasynuclein-A53T
catalog :
40823
citations: 27
Reference
Park J, Choe K, Lee H, Park T, Kim M. Neuroprotective effects of osmotin in Parkinson's disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways. J Biomed Sci. 2023;30:66 pubmed publisher
Russo T, Kolisnyk B, Bs A, Kim T, Martin J, Plessis Belair J, et al. The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons. bioRxiv. 2023;: pubmed publisher
Lee H, Choe K, Park J, Khan A, Kim M, Park T, et al. O-Cyclic Phytosphingosine-1-Phosphate Protects against Motor Dysfunctions and Glial Cell Mediated Neuroinflammation in the Parkinson's Disease Mouse Models. Antioxidants (Basel). 2022;11: pubmed publisher
Kulkarni V, Stempel M, Anand A, Sidibe D, Maday S. Retrograde Axonal Autophagy and Endocytic Pathways Are Parallel and Separate in Neurons. J Neurosci. 2022;42:8524-8541 pubmed publisher
Wrobel L, Hill S, Djajadikerta A, Fernandez Estévez M, Karabiyik C, Ashkenazi A, et al. Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance. Nat Commun. 2022;13:4146 pubmed publisher
Potdar C, Kaushal A, Raj A, Mallick R, Datta I. Reduction of phosphorylated α-synuclein through downregulation of casein kinase 2α alleviates dopaminergic-neuronal function. Biochem Biophys Res Commun. 2022;615:43-48 pubmed publisher
Peinado J, Chaplot K, Jarvela T, Barbieri E, Shorter J, Lindberg I. Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. ACS Chem Neurosci. 2022;13:1651-1665 pubmed publisher
Qiu W, Ai W, Zhu F, Zhang Y, Guo M, Law B, et al. Polygala saponins inhibit NLRP3 inflammasome-mediated neuroinflammation via SHP-2-Mediated mitophagy. Free Radic Biol Med. 2021;179:76-94 pubmed publisher
Chen J, Mao K, Yu H, Wen Y, She H, Zhang H, et al. p38-TFEB pathways promote microglia activation through inhibiting CMA-mediated NLRP3 degradation in Parkinson's disease. J Neuroinflammation. 2021;18:295 pubmed publisher
Wang C, Lau C, Ma F, Zheng C. Genome-wide screen identifies curli amyloid fibril as a bacterial component promoting host neurodegeneration. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Yuste Checa P, Trinkaus V, Riera Tur I, Imamoglu R, Schaller T, Wang H, et al. The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model. Nat Commun. 2021;12:4863 pubmed publisher
Trinkaus V, Riera Tur I, Martinez Sanchez A, Bäuerlein F, Guo Q, Arzberger T, et al. In situ architecture of neuronal α-Synuclein inclusions. Nat Commun. 2021;12:2110 pubmed publisher
Jia R, Bonifacino J. The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. J Biol Chem. 2021;296:100405 pubmed publisher
Mao K, Chen J, Yu H, Li H, Ren Y, Wu X, et al. Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes α-synuclein degradation via transcription factor EB-dependent autophagy in mutant α-synucleinA53T model of Parkinson's disease. Aging Cell. 2020;19:e13163 pubmed publisher
Furlong R, O Keeffe G, O Neill C, Sullivan A. Alterations in α-synuclein and PINK1 expression reduce neurite length and induce mitochondrial fission and Golgi fragmentation in midbrain neurons. Neurosci Lett. 2020;720:134777 pubmed publisher
Gui C, Ren Y, Chen J, Wu X, Mao K, Li H, et al. p38 MAPK-DRP1 signaling is involved in mitochondrial dysfunction and cell death in mutant A53T α-synuclein model of Parkinson's disease. Toxicol Appl Pharmacol. 2019;388:114874 pubmed publisher
D Agostino M, Scerra G, Cannata Serio M, Caporaso M, Bonatti S, Renna M. Unconventional secretion of α-Crystallin B requires the Autophagic pathway and is controlled by phosphorylation of its serine 59 residue. Sci Rep. 2019;9:16892 pubmed publisher
Jia R, Bonifacino J. Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. elife. 2019;8: pubmed publisher
Mazzocchi M, Wyatt S, Mercatelli D, Morari M, Morales Prieto N, Collins L, et al. Gene Co-expression Analysis Identifies Histone Deacetylase 5 and 9 Expression in Midbrain Dopamine Neurons and as Regulators of Neurite Growth via Bone Morphogenetic Protein Signaling. Front Cell Dev Biol. 2019;7:191 pubmed publisher
Coudert L, Nonaka T, Bernard E, Hasegawa M, Schaeffer L, Leblanc P. Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. Cell Mol Life Sci. 2019;76:2615-2632 pubmed publisher
Shaltouki A, Hsieh C, Kim M, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol. 2018;136:607-620 pubmed publisher
Chen J, Ren Y, Gui C, Zhao M, Wu X, Mao K, et al. Phosphorylation of Parkin at serine 131 by p38 MAPK promotes mitochondrial dysfunction and neuronal death in mutant A53T α-synuclein model of Parkinson's disease. Cell Death Dis. 2018;9:700 pubmed publisher
Jo M, Ikram M, Jo M, Yoo L, Chung K, Nah S, et al. Gintonin Mitigates MPTP-Induced Loss of Nigrostriatal Dopaminergic Neurons and Accumulation of α-Synuclein via the Nrf2/HO-1 Pathway. Mol Neurobiol. 2019;56:39-55 pubmed publisher
Kumar R, Bavi R, Jo M, Arulalapperumal V, Baek A, Rampogu S, et al. New compounds identified through in silico approaches reduce the α-synuclein expression by inhibiting prolyl oligopeptidase in vitro. Sci Rep. 2017;7:10827 pubmed publisher
Button R, Roberts S, Willis T, Hanemann C, Luo S. Accumulation of autophagosomes confers cytotoxicity. J Biol Chem. 2017;292:13599-13614 pubmed publisher
Dieriks B, Park T, Fourie C, Faull R, Dragunow M, Curtis M. α-synuclein transfer through tunneling nanotubes occurs in SH-SY5Y cells and primary brain pericytes from Parkinson's disease patients. Sci Rep. 2017;7:42984 pubmed publisher
Furlong R, Narain Y, Rankin J, Wyttenbach A, Rubinsztein D. Alpha-synuclein overexpression promotes aggregation of mutant huntingtin. Biochem J. 2000;346 Pt 3:577-81 pubmed
product information
Catalog Number :
40823
Product Name :
EGFP-alphasynuclein-A53T
article :
doi10.1042/0264-6021:3460577
id5941
pubmed_id10698681
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Alpha-synuclein
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutationA53T (aggregates more rapidly than the wild-type form when the recombinant proteins are 'aged' at 37 degree C)
nameSNCA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1372
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA