This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-alphasynuclein-WT
catalog :
40822
citations: 28
Reference
Yayli G, Bernardini A, Mendoza Sanchez P, Scheer E, Damilot M, Essabri K, et al. ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct. Cell Rep. 2023;42:113099 pubmed publisher
Torrino S, Oldham W, Tejedor A, Burgos I, Rachedi N, Fraissard K, et al. Mechano-dependent sorbitol accumulation supports biomolecular condensate. bioRxiv. 2023;: pubmed publisher
Kulkarni V, Stempel M, Anand A, Sidibe D, Maday S. Retrograde Axonal Autophagy and Endocytic Pathways Are Parallel and Separate in Neurons. J Neurosci. 2022;42:8524-8541 pubmed publisher
Peinado J, Chaplot K, Jarvela T, Barbieri E, Shorter J, Lindberg I. Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. ACS Chem Neurosci. 2022;13:1651-1665 pubmed publisher
Lee J, Xu Y, Saidi L, Xu M, ZINSMAIER K, Ye Y. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy. 2022;:1-20 pubmed publisher
McCarthy E, Barron A, Morales Prieto N, Mazzocchi M, McCarthy C, Collins L, et al. Gene Co-expression Analysis of the Human Substantia Nigra Identifies ZNHIT1 as an SNCA Co-expressed Gene that Protects Against α-Synuclein-Induced Impairments in Neurite Growth and Mitochondrial Dysfunction in SH-SY5Y Cells. Mol Neurobiol. 2022;59:2745-2757 pubmed publisher
Vogiatzis S, Celestino M, Trevisan M, Magro G, Del Vecchio C, Erdengiz D, et al. Lentiviral Vectors Expressing Chimeric NEDD4 Ubiquitin Ligases: An Innovative Approach for Interfering with Alpha-Synuclein Accumulation. Cells. 2021;10: pubmed publisher
Anantha J, Goulding S, Tuboly E, O Mahony A, Moloney G, Lomansey G, et al. NME1 Protects Against Neurotoxin-, α-Synuclein- and LRRK2-Induced Neurite Degeneration in Cell Models of Parkinson's Disease. Mol Neurobiol. 2021;: pubmed publisher
Wang C, Lau C, Ma F, Zheng C. Genome-wide screen identifies curli amyloid fibril as a bacterial component promoting host neurodegeneration. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Cao L, Xiong S, Wu Z, Ding L, Zhou Y, Sun H, et al. Anti-Na+/K+-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na+/K+-ATPase α1-dependent autophagy. Sci Adv. 2021;7: pubmed publisher
Chen V, Moncalvo M, Tringali D, Tagliafierro L, Shriskanda A, Ilich E, et al. The mechanistic role of alpha-synuclein in the nucleus: impaired nuclear function caused by familial Parkinson's disease SNCA mutations. Hum Mol Genet. 2020;29:3107-3121 pubmed publisher
Sn S, Pandurangi J, Murumalla R, Dj V, Garimella L, Acharya A, et al. Small molecule modulator of aggrephagy regulates neuroinflammation to curb pathogenesis of neurodegeneration. EBioMedicine. 2019;50:260-273 pubmed publisher
Taguchi Y, Gorenberg E, Nagy M, Thrasher D, Fenton W, Volpicelli Daley L, et al. Hsp110 mitigates α-synuclein pathology in vivo. Proc Natl Acad Sci U S A. 2019;116:24310-24316 pubmed publisher
Mazzocchi M, Wyatt S, Mercatelli D, Morari M, Morales Prieto N, Collins L, et al. Gene Co-expression Analysis Identifies Histone Deacetylase 5 and 9 Expression in Midbrain Dopamine Neurons and as Regulators of Neurite Growth via Bone Morphogenetic Protein Signaling. Front Cell Dev Biol. 2019;7:191 pubmed publisher
Osuagwu N, Dölle C, Tzoulis C. Poly-ADP-ribose assisted protein localization resolves that DJ-1, but not LRRK2 or α-synuclein, is localized to the mitochondrial matrix. PLoS ONE. 2019;14:e0219909 pubmed publisher
Coudert L, Nonaka T, Bernard E, Hasegawa M, Schaeffer L, Leblanc P. Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. Cell Mol Life Sci. 2019;76:2615-2632 pubmed publisher
Mann J, Gleixner A, Mauna J, Gomes E, DeChellis Marks M, Needham P, et al. RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43. Neuron. 2019;102:321-338.e8 pubmed publisher
Shaltouki A, Hsieh C, Kim M, Wang X. Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models. Acta Neuropathol. 2018;136:607-620 pubmed publisher
Suresh S, Chavalmane A, Pillai M, Ammanathan V, Vidyadhara D, Yarreiphang H, et al. Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective. Front Mol Neurosci. 2018;11:109 pubmed publisher
Taguchi Y, Liu J, Ruan J, Pacheco J, Zhang X, Abbasi J, et al. Glucosylsphingosine Promotes α-Synuclein Pathology in Mutant GBA-Associated Parkinson's Disease. J Neurosci. 2017;37:9617-9631 pubmed publisher
Amorim L, Gaspar R, Pereira P, ÄŒernigoj U, Sousa F, Queiroz J, et al. Chromatographic HPV-16 E6/E7 plasmid vaccine purification employing L-histidine and 1-benzyl-L-histidine affinity ligands. Electrophoresis. 2017;38:2975-2980 pubmed publisher
Yasui T, Uezono N, Nakashima H, Noguchi H, Matsuda T, Noda Andoh T, et al. Hypoxia Epigenetically Confers Astrocytic Differentiation Potential on Human Pluripotent Cell-Derived Neural Precursor Cells. Stem Cell Reports. 2017;8:1743-1756 pubmed publisher
Suresh S, Chavalmane A, Dj V, Yarreiphang H, Rai S, Paul A, et al. A novel autophagy modulator 6-Bio ameliorates SNCA/?-synuclein toxicity. Autophagy. 2017;13:1221-1234 pubmed publisher
Liu S, Kiyoi T, Takemasa E, Maeyama K. Intra-articular lentivirus-mediated gene therapy targeting CRACM1 for the treatment of collagen-induced arthritis. J Pharmacol Sci. 2017;133:130-138 pubmed publisher
Dieriks B, Park T, Fourie C, Faull R, Dragunow M, Curtis M. α-synuclein transfer through tunneling nanotubes occurs in SH-SY5Y cells and primary brain pericytes from Parkinson's disease patients. Sci Rep. 2017;7:42984 pubmed publisher
Bickle L, Hopwood J, Karageorgos L. Analysis of sheep ?-synuclein provides a molecular strategy for the reduction of fibrillation. Biochim Biophys Acta Proteins Proteom. 2017;1865:261-273 pubmed publisher
Emanuele M, Esposito A, Camerini S, Antonucci F, Ferrara S, Seghezza S, et al. Exogenous Alpha-Synuclein Alters Pre- and Post-Synaptic Activity by Fragmenting Lipid Rafts. EBioMedicine. 2016;7:191-204 pubmed publisher
Furlong R, Narain Y, Rankin J, Wyttenbach A, Rubinsztein D. Alpha-synuclein overexpression promotes aggregation of mutant huntingtin. Biochem J. 2000;346 Pt 3:577-81 pubmed
product information
Catalog Number :
40822
Product Name :
EGFP-alphasynuclein-WT
article :
doi10.1042/0264-6021:3460577
id5941
pubmed_id10698681
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that EcoRI is not a unique site. The alpha-synuclein insert contains an EcoRI site in the coding sequence.
origin :
37
pi :
alt_names
Alpha-synuclein
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNACP, PARK1, PARK4, PD1
geneSNCA
id6622
genbank_ids
mutation
nameSNCA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1372
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA