This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCL-HA-MAPKK1-8E (K97M)
catalog :
40811
citations: 2
Reference
Tsanov K, Pearson D, Wu Z, Han A, Triboulet R, Seligson M, et al. LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency. Nat Cell Biol. 2017;19:60-67 pubmed publisher
Mansour S, Matten W, Hermann A, Candia J, Rong S, Fukasawa K, et al. Transformation of mammalian cells by constitutively active MAP kinase kinase. Science. 1994;265:966-70 pubmed
product information
Catalog Number :
40811
Product Name :
pMCL-HA-MAPKK1-8E (K97M)
article :
doi10.1126/science.8052857
id5935
pubmed_id8052857
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutationSee attached map & info
backbone_originInvitrogen
backbone_size10200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
MKK1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
NM_002755
mutationK97M
nameMAP2K1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
1371
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA