This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCL-HA-MAPKK1-11A55B [S218E/S222D]
catalog :
40809
citations: 3
Reference
Greaves S, Peterson J, Torres R, Pelanda R. Activation of the MEK-ERK Pathway Is Necessary but Not Sufficient for Breaking Central B Cell Tolerance. Front Immunol. 2018;9:707 pubmed publisher
Huang H, Joseph L, Gurin M, Thorp E, Morrow J. Extracellular signal-regulated kinase activation during cardiac hypertrophy reduces sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) transcription. J Mol Cell Cardiol. 2014;75:58-63 pubmed publisher
Mansour S, Matten W, Hermann A, Candia J, Rong S, Fukasawa K, et al. Transformation of mammalian cells by constitutively active MAP kinase kinase. Science. 1994;265:966-70 pubmed
product information
Catalog Number :
40809
Product Name :
pMCL-HA-MAPKK1-11A55B [S218E/S222D]
article :
doi10.1126/science.8052857
id5935
pubmed_id8052857
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutationSee attached map & info
backbone_originInvitrogen
backbone_size10200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
MKK1
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamH1
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
NM_002755
mutationS218E, S222D
nameMAP2K1
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHA
resistance markers :
1371
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA