hGFAP-Cre | Addgene 40591 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

hGFAP-Cre

catalog :

40591

citations: 7

Reference
Tabata H, Sasaki M, Agetsuma M, Sano H, Hirota Y, Miyajima M, et al. Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex. Nat Commun. 2022;13:6571 pubmed publisher
Puls B, Ding Y, Zhang F, Pan M, Lei Z, Pei Z, et al. Regeneration of Functional Neurons After Spinal Cord Injury via in situ NeuroD1-Mediated Astrocyte-to-Neuron Conversion. Front Cell Dev Biol. 2020;8:591883 pubmed publisher
Zhang L, Lei Z, Guo Z, Pei Z, Chen Y, Zhang F, et al. Development of Neuroregenerative Gene Therapy to Reverse Glial Scar Tissue Back to Neuron-Enriched Tissue. Front Cell Neurosci. 2020;14:594170 pubmed publisher
Chen Y, Ma N, Pei Z, Wu Z, Do Monte F, KEEFE S, et al. A NeuroD1 AAV-Based Gene Therapy for Functional Brain Repair after Ischemic Injury through In Vivo Astrocyte-to-Neuron Conversion. Mol Ther. 2020;28:217-234 pubmed publisher
Li M, Li Z, Yao Y, Jin W, Wood K, Liu Q, et al. Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity. Proc Natl Acad Sci U S A. 2017;114:E396-E405 pubmed publisher
Hamilton L, Dufresne M, JoppÃ© S, Petryszyn S, Aumont A, Calon F, et al. Aberrant Lipid Metabolism in the Forebrain Niche Suppresses Adult Neural Stem Cell Proliferation in an Animal Model of Alzheimer's Disease. Cell Stem Cell. 2015;17:397-411 pubmed publisher
Zhuo L, Theis M, Alvarez Maya I, Brenner M, Willecke K, Messing A. hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo. Genesis. 2001;31:85-94 pubmed

product information

Catalog Number :

40591

Product Name :

hGFAP-Cre

article :

doi	10.1002/gene.10008
id	5886
pubmed_id	11668683

bacterial resistance :

Ampicillin

cloning :

backbone

pgfa2-lac2

backbone_mutation

LacZ coding region of pgfa2-lac2 replaced by a nuclear-targeted Cre coding sequence.

backbone_origin

Michael Brenner (PMID: 7952266)

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Bacterial Expression

Mouse Targeting

Cre/Lox

growth notes :

This plasmid encodes a transgene to express the Cre recombinase in astrocytes of transgenic mice. A DNA fragment encoding the Cre recombinase was inserted into an expression cassette containing the gfa2 promoter (Besnard et al., 1991), a 2.2-kb 5' flanking region from the human GFAP (hGFAP) gene. The nuclear targeting signal from the SV40 large T antigen was inserted at the amino terminal end of the coding region, because it reportedly increases the efficiency of DNA excision by the recombinase (Gu et al., 1993). A heterologous intron and polyadenylation signal were provided by sequences from the mouse protamine 1 gene at the 3' end. The hGFAP-cre transgene was constructed by excising the lacZ coding region from pgfa2-lac2 (Brenner et al., 1994) by BamHI digestion, and replacing it by blunt end ligation with a nuclear-targeted Cre coding sequence. The latter was obtained as a 1.1-kb SalI-MluI fragment from plasmid pTZ19RCreNLS, which was kindly provided by Drs. Raul Torres and Klaus Rajewsky. Both junctions were verified by DNA sequencing.

origin :

pi :

alt_names

CreNLS

hGFAP-Promoter-Cre-MP-1

Tg95

cloning

clone_method	Restriction Enzyme
cloning_site_3	MluI
cloning_site_5	SalI
promoter	hGFAP promoter
sequencing_primer_3
sequencing_primer_5	GFAPpro-F (5'-ACTCCTTCATAAAGCCCTCG-3')
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	ALXDRD
gene	GFAP
id	2670

genbank_ids

mutation

Construct contains a nuclear-targeted Cre recombinase followed by a heterologous mouse intron and polyadenylation signal, all under the control of the gfa2 promoter

name

Cre recombinase

shRNA_sequence

size

1100

species

9606

Homo sapiens

10090

Mus musculus

Phage