BCL6-H2Kk-RV (H2K-BCL6) | Addgene 40352 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

BCL6-H2Kk-RV (H2K-BCL6)

catalog :

40352

citations: 1

Reference
Mondal A, Sawant D, Dent A. Transcriptional repressor BCL6 controls Th17 responses by controlling gene expression in both T cells and macrophages. J Immunol. 2010;184:4123-32 pubmed publisher

product information

Catalog Number :

40352

Product Name :

BCL6-H2Kk-RV (H2K-BCL6)

article :

doi	10.4049/jimmunol.0901242
id	5871
pubmed_id	20212093

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV2.1-H2Kk

backbone_mutation

Mouse H2K-k cDNA cloned after MSCV2.1 IRES

backbone_origin

Mark Kaplan

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

A MSCV2.1 retroviral vector was modified by cloning of mouse H2K-k cDNA after the IRES sequence to make MSCV2.1-H2Kk. MSCV2.1-H2Kk was obtained from Dr. Mark Kaplan. MSCV2.1-H2Kk was then cut with BglII and a human BCL6 cDNA with BamHI ends was cloned into the BglII site.

origin :

pi :

alt_names

BCL-6

B-cell CLL/lymphoma 6

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI/BglII
cloning_site_5	BglII/BamHI
promoter
sequencing_primer_3	IRES-R2 (gacggcaatatggtggaaa)
sequencing_primer_5	pLXSN_5
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
gene	BCL6
id	604

genbank_ids

NG_007149.1

mutation

name

BCL6

shRNA_sequence

size

species

9606

Homo sapiens