This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIEG3-NFAT2
catalog :
40351
citations: 1
Reference
Wang Z, Kusam S, Munugalavadla V, Kapur R, Brutkiewicz R, Dent A. Regulation of Th2 cytokine expression in NKT cells: unconventional use of Stat6, GATA-3, and NFAT2. J Immunol. 2006;176:880-8 pubmed
product information
Catalog Number :
40351
Product Name :
pMIEG3-NFAT2
article :
doi
id5869
pubmed_id16393972
bacterial resistance :
Ampicillin
cloning :
backbonepMIEG3
backbone_mutation
backbone_originDavid A. Williams (PMID: 10961859)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The NFAT2-expressing retroviruses were constructed by inserting blunt-ended NFAT2 cDNA from pSH160C into the retroviral vector pMIEG3 via the blunt-ended XhoI site.
origin :
37
pi :
alt_names
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3IRES-R2 (gacggcaatatggtggaaa)
sequencing_primer_5pLXSN_5
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesNF-ATC, NF-ATc1.2, NFAT2, NFATc
geneNFATC1
id4772
genbank_ids
NG_029226.1
mutation
nameNFAT2
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on backbone
tagIRES eGFP
resistance markers :
1357
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA