pMIEG3-JunDN | Addgene 40350 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMIEG3-JunDN

catalog :

40350

citations: 5

Reference
Jameson N, Ma J, Benitez J, Izurieta A, Han J, Mendez R, et al. Intron 1-Mediated Regulation of EGFR Expression in EGFR-Dependent Malignancies Is Mediated by AP-1 and BET Proteins. Mol Cancer Res. 2019;17:2208-2220 pubmed publisher
Gill M, Christova T, Zhang Y, Gregorieff A, Zhang L, Narimatsu M, et al. A feed forward loop enforces YAP/TAZ signaling during tumorigenesis. Nat Commun. 2018;9:3510 pubmed publisher
Gurdziel K, Vogt K, Walton K, Schneider G, Gumucio D. Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun. Dev Dyn. 2016;245:614-26 pubmed publisher
Liu X, Zhang J, Li J, Volk A, Breslin P, Zhang J, et al. The synergistic repressive effect of NF-ÎºB and JNK inhibitor on the clonogenic capacity of Jurkat leukemia cells. PLoS ONE. 2014;9:e115490 pubmed publisher
Wang Z, Sato H, Kusam S, Sehra S, Toney L, Dent A. Regulation of IL-10 gene expression in Th2 cells by Jun proteins. J Immunol. 2005;174:2098-105 pubmed

product information

Catalog Number :

40350

Product Name :

pMIEG3-JunDN

article :

doi
id	5870
pubmed_id	15699140

bacterial resistance :

Ampicillin

cloning :

backbone

pMIEG3

backbone_mutation

backbone_origin

David A. Williams (PMID: 10961859)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

The antisense primer adds a hemagglutinin tag to the C terminus of c-Jun. The PCR product was then cut with EcoRI and cloned into the retroviral vector pMIEG3.
5-AAAAAA-GAATTC-TCAGCTGGCATAGTCAGGCACG
TCATAAGGATAGCTAAATGTTTGCAAC-3.
The Jun dominant negative vector was made by first preparing a version of c-Jun with the first 122 aa deleted. This cDNA was amplified by PCR from human c-Jun with the following primers: sense, 5-AAAAAA-GAATTC-ATGACTAGCCAGAACACGCTGCCCAGCGTC-3; antisense,

origin :

pi :

alt_names

JunDN

c-Jun

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	IRES-R2 (gacggcaatatggtggaaa)
sequencing_primer_5	pLXSN_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AP-1, AP1, c-Jun, cJUN, p39
gene	JUN
id	3725

genbank_ids

mutation

first 122 amino acids deleted

name

Jun dominant negative

shRNA_sequence

size

species

9606

Homo sapiens