CXN-BCL-6 ZF (pCXN2-BCL6-ZFdel) | Addgene 40347 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CXN-BCL-6 ZF (pCXN2-BCL6-ZFdel)

catalog :

40347

citations: 1

Reference
Vasanwala F, Kusam S, Toney L, Dent A. Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. J Immunol. 2002;169:1922-9 pubmed

product information

Catalog Number :

40347

Product Name :

CXN-BCL-6 ZF (pCXN2-BCL6-ZFdel)

article :

doi
id	5860
pubmed_id	12165517

bacterial resistance :

Ampicillin

cloning :

backbone

pCXN2

backbone_mutation

backbone_origin

Niwa, Yamamura, and Miyazaki (PMID: 1660837)

backbone_size

5200

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The CXN-BCL-6 ZF deletion was made by digesting pBS-BCL-6FL with AspI, which cleaves a unique site in the human BCL-6 cDNA 45 bp before the start of the ZF domain, and HindIII, which cleaves the pBS vector. The digested plasmid was separated from the smaller deleted fragment, and ligated. The truncated cDNA was then cloned into pCXN2 via SalI and XhoI.

origin :

pi :

alt_names

BCL-6

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	pCAG
sequencing_primer_3	Bglob-pA-R (ttttggcagagggaaaaaga)
sequencing_primer_5	pCAG-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
gene	BCL6
id	604

genbank_ids

mutation

Contains aa1-504; ZF domain deleted (see note below)

name

BCL-6 ZF

shRNA_sequence

size

species

9606

Homo sapiens