This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCIneoFH human RASSF6
catalog :
40344
citations: 1
Reference
Ikeda M, Kawata A, Nishikawa M, Tateishi Y, Yamaguchi M, Nakagawa K, et al. Hippo pathway-dependent and -independent roles of RASSF6. Sci Signal. 2009;2:ra59 pubmed publisher
product information
Catalog Number :
40344
Product Name :
pCIneoFH human RASSF6
article :
doi10.1126/scisignal.2000300
id5350
pubmed_id19797269
bacterial resistance :
Ampicillin
cloning :
backbonepCI-neo (modified)
backbone_mutationpCI-neo vector was modified to include FLAG and His tags N-terminal to MCS. The resulting vector was named pCIneoFLAG-His6(FH).
backbone_originPromega
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pCIneoFH vector has the sequence NheI-FLAG-His6-EcoRI-MluI-XbaI-SalI-SmaI-NotI. A digest with NheI and SalI will excise the insert with tags.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5MluI
promoterCMV
sequencing_primer_3T3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneRASSF6
id166824
genbank_ids
NP_001257320.1
mutation
nameRASSF6
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
locationN terminal on backbone
tagHis
resistance markers :
1244
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA