Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

catalog :

40341

citations: 3

Reference
Lee H, Huang D, Chang H, Lin C, Ren W, Dai Y, et al. Blimp-1 Upregulation by Multiple Ligands via EGFR Transactivation Inhibits Cell Migration in Keratinocytes and Squamous Cell Carcinoma. Front Pharmacol. 2022;13:763678 pubmed publisher
Meng J, Zhang W, Wang C, Xiong S, Wang Q, Li H, et al. The dipeptidyl peptidase (DPP)-4 inhibitor trelagliptin inhibits IL-1β-induced endothelial inflammation and monocytes attachment. Int Immunopharmacol. 2020;89:106996 pubmed publisher
Vasanwala F, Kusam S, Toney L, Dent A. Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. J Immunol. 2002;169:1922-9 pubmed

product information

Catalog Number :

40341

Product Name :

Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

article :

doi
id	5860
pubmed_id	12165517

bacterial resistance :

Ampicillin

cloning :

backbone

pGL3-basic

backbone_mutation

backbone_origin

Promega

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

This mutant AP-1 site promoter, Mut.Blimp-1pGL3, was constructed by replacing the two AP-1 sites at -1813 and -1647 with BamHI and EcoRI restriction enzyme sites, respectively. Addgene sequencing results have determined that this is a truncated promoter of ~400bp

origin :

pi :

alt_names

PRDM1 promoter (mutated)

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	KpnI
promoter	Blimp1
sequencing_primer_3	LucNrev
sequencing_primer_5	RVprimer3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BLIMP1, PRDI-BF1
gene	PRDM1
id	639

genbank_ids

mutation

Two AP-1 sites at -1813 and -1647 replaced with BamHI and EcoRI restriction enzyme sites, respectively

name

Blimp1 promoter (mutated)

shRNA_sequence

size

species

9606

Homo sapiens