This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)
catalog :
40340
citations: 2
| Reference |
|---|
Vasanwala F, Kusam S, Toney L, Dent A. Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene. J Immunol. 2002;169:1922-9 pubmed
|
product information
Catalog Number :
40340
Product Name :
Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)
article :
| doi | |
| id | 5860 |
| pubmed_id | 12165517 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pGL3-basic | ||
| backbone_mutation | |||
| backbone_origin | Promega | ||
| backbone_size | 4818 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
A 2000-bp region of the human Blimp-1 promoter was isolated from human (Raji cell) genomic DNA by PCR using 5-ATTTTAGGGTTGCCTGGCTAAGCC-3 as the forward and 5-GTCACGGCAGCACTTTGTCTGTGT-3 as the reverse primers. The PCR product obtained was ligated into the pcR2.1 TOPO cloning vector (Invitrogen, Carlsbad, CA) and then subcloned into pGL3-basic (Promega, Madison, WI) using SacI and XhoI sites. The promoter construct was sequenced to verify that the proper sequence was amplified.
origin :
37
pi :
|
resistance markers :
| 1357 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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