This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIEG3-BCL6
catalog :
40339
citations: 2
Reference
Vacchio M, Ciucci T, Gao Y, Watanabe M, Balmaceno Criss M, McGinty M, et al. A Thpok-Directed Transcriptional Circuitry Promotes Bcl6 and Maf Expression to Orchestrate T Follicular Helper Differentiation. Immunity. 2019;51:465-478.e6 pubmed publisher
Toney L, Cattoretti G, Graf J, Merghoub T, Pandolfi P, Dalla Favera R, et al. BCL-6 regulates chemokine gene transcription in macrophages. Nat Immunol. 2000;1:214-20 pubmed
product information
Catalog Number :
40339
Product Name :
pMIEG3-BCL6
article :
doi10.1038/79749
id5858
pubmed_id10973278
bacterial resistance :
Ampicillin
cloning :
backbonepMIEG3
backbone_mutation
backbone_originDavid A. Williams (PMID: 10961859)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The pMIEG3 plasmid was obtained from D.A. Williams (Indiana University School of Medicine). pMIEG3 is a murine stem cell virus (MSCV) retrovirus construct in which the gene encoding GFP is driven by the MSCV long terminal repeat. pMIEG3 contains an IRES that allows coexpression of the GFP gene and a second gene. The human BCL-6 cDNA with an optimized translation initiation codon (obtained from L.M. Staudt, NCI, NIH) was cloned into pMIEG3 via NotI and XhoI restriction sites.
origin :
37
pi :
alt_names
BCL-6
B-cell CLL/lymphoma 6
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NotI
promoter
sequencing_primer_3IRES-R2 (gacggcaatatggtggaaa)
sequencing_primer_5pLXSN_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCL5, BCL6A, LAZ3, ZBTB27, ZNF51
geneBCL6
id604
genbank_ids
NG_007149.1
mutationoptimized translation initiation codon
nameBCL6
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagIRES eGFP
plasmid copy :
Human BCL-6 cDNA with optimized translation initiation codon obtained from L.M. Staudt, NCI, NIH.
resistance markers :
1357
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA