pCGN-BCL-6 (pCGN-BCL6-delta) | Addgene 40338 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCGN-BCL-6 (pCGN-BCL6-delta)

catalog :

40338

citations: 1

Reference
Toney L, Cattoretti G, Graf J, Merghoub T, Pandolfi P, Dalla Favera R, et al. BCL-6 regulates chemokine gene transcription in macrophages. Nat Immunol. 2000;1:214-20 pubmed

product information

Catalog Number :

40338

Product Name :

pCGN-BCL-6 (pCGN-BCL6-delta)

article :

doi	10.1038/79749
id	5858
pubmed_id	10973278

bacterial resistance :

Ampicillin

cloning :

backbone

pCGN

backbone_mutation

backbone_origin

Masafumi Tanaka and Winship Herr (PMID: 2302733)

backbone_size

7500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The expression plasmid pCGN-BCL-6 was constructed by digesting the BCL-6 cDNA with EcoRI, deleting the 3' region, and cloning the remaining cDNA into pCGN. The BCL-6 is missing four of six zinc fingers and does not bind to a consensus BCL-6 probe in a gel shift assay (A. L. Dent, unpublished data). Note, the cloning and ligation adds the following residues after aa595 of BCL6: RYQAYRYRRPGYR

origin :

pi :

alt_names

BCL6

BCL-6

BCL6

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3	Bglob-intron-R (TTTGCCCCCTCCATATAACA)
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCL5, BCL6A, LAZ3, ZBTB27, ZNF51
gene	BCL6
id	604

genbank_ids

NM_001706.4

NM_001130845.1

mutation

Missing four of six zinc fingers (see note below); contains aa 1-595

name

BCL-6

shRNA_sequence

size

species

9606

Homo sapiens