This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHLUM
catalog :
40276
citations: 7
Reference
Zelezniak A, Vowinckel J, Capuano F, Messner C, Demichev V, Polowsky N, et al. Machine Learning Predicts the Yeast Metabolome from the Quantitative Proteome of Kinase Knockouts. Cell Syst. 2018;7:269-283.e6 pubmed publisher
Mülleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M. Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities. F1000Res. 2016;5:2351 pubmed publisher
Mülleder M, Calvani E, Alam M, Wang R, Eckerstorfer F, Zelezniak A, et al. Functional Metabolomics Describes the Yeast Biosynthetic Regulome. Cell. 2016;167:553-565.e12 pubmed publisher
Kumar A, Birnbaum M, Patel D, Morgan W, Singh J, Barrientos A, et al. Posttranslational arginylation enzyme Ate1 affects DNA mutagenesis by regulating stress response. Cell Death Dis. 2016;7:e2378 pubmed publisher
Campbell K, Vowinckel J, Keller M, Ralser M. Methionine Metabolism Alters Oxidative Stress Resistance via the Pentose Phosphate Pathway. Antioxid Redox Signal. 2016;24:543-7 pubmed publisher
Campbell K, Vowinckel J, Mülleder M, Malmsheimer S, Lawrence N, Calvani E, et al. Self-establishing communities enable cooperative metabolite exchange in a eukaryote. elife. 2015;4: pubmed publisher
Mülleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver S, et al. A prototrophic deletion mutant collection for yeast metabolomics and systems biology. Nat Biotechnol. 2012;30:1176-8 pubmed publisher
product information
Catalog Number :
40276
Product Name :
pHLUM
article :
doi10.1038/nbt.2442
id6126
pubmed_id23222782
bacterial resistance :
Ampicillin
cloning :
backbonepRS313
backbone_mutation
backbone_originATCC, Number: 77142
backbone_size4967
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
origin :
37
pi :
alt_names
YCL018W
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5AscI
promoterendogenous LEU2
sequencing_primer_3TAATACGACTCACTATAGGG
sequencing_primer_5AACAAAGGAACCTAGAGGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYCL018W
geneLEU2
id850342
genbank_ids
NM_001178665.1
mutation
nameLEU2
shRNA_sequence
size2116
species
4932
Saccharomyces cerevisiae
tags
alt_names
YEL021W
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5SphI
promoterendogenous URA3
sequencing_primer_3GGCCTCTAGGTTCCTTTGTT
sequencing_primer_5GATTCGGTAATCTCCGAAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL021W
geneURA3
id856692
genbank_ids
NM_001178836
mutation
nameURA3
shRNA_sequence
size1096
species
4932
Saccharomyces cerevisiae
tags
alt_names
YLR303W
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5SphI
promoterendogenous MET15/17
sequencing_primer_3TGCTTCCGGCTCCTATGTTGTG
sequencing_primer_5ATCGCATGCGCCATCCTCATGAAAACTGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYLR303W, MET15, MET25
geneMET17
id851010
genbank_ids
NM_001182191
mutation
nameMET15/17
shRNA_sequence
size1620
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
genes were amplified by PCR from plasmids pRS425 (LEU2, ATCC Number:77106), p426GPD (URA3, ATCC Number:87361) and pRS411 (MET15/17, ATCC Number:87474)
resistance markers :
404
tags :
High Copy
terms :
LEU2
URA3
HIS3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA