This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-Q74
catalog :
40262
citations: 38
Reference
Llamas E, Koyuncu S, Lee H, Wehrmann M, Gutiérrez García R, Dunken N, et al. In planta expression of human polyQ-expanded huntingtin fragment reveals mechanisms to prevent disease-related protein aggregation. Nat Aging. 2023;3:1345-1357 pubmed publisher
Lee D, Choi C, Sanders D, Beckers L, Riback J, Brangwynne C, et al. Size distributions of intracellular condensates reflect competition between coalescence and nucleation. Nat Phys. 2023;19:586-596 pubmed publisher
Pereira C, Medaglia N, Ureshino R, Bincoletto C, Antonioli M, Fimia G, et al. NAADP-Evoked Ca2+ Signaling Leads to Mutant Huntingtin Aggregation and Autophagy Impairment in Murine Astrocytes. Int J Mol Sci. 2023;24: pubmed publisher
Mansky R, Greguske E, Yu D, Zarate N, Intihar T, Tsai W, et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease. Cell Rep. 2023;42:112198 pubmed publisher
Joshi B, Zuhorn I. Preparation of chaperone-loaded neural stem cell-derived extracellular vesicles to reduce protein aggregation in Huntington's disease cellular models. STAR Protoc. 2023;4:102134 pubmed publisher
Haver H, Wedemeyer M, Butcher E, Peterson F, Volkman B, Scaglione K. Mechanistic Insight into the Suppression of Polyglutamine Aggregation by SRCP1. ACS Chem Biol. 2023;18:549-560 pubmed publisher
Joshi B, Garcia Romeu H, Aliyandi A, de Vries M, Zuhorn I. DNAJB6-Containing Extracellular Vesicles as Chaperone Delivery Systems: A Proteomic Analysis. Pharmaceutics. 2022;14: pubmed publisher
Ahat E, Bui S, Zhang J, da Veiga Leprevost F, Sharkey L, Reid W, et al. GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin. J Biol Chem. 2022;:102219 pubmed publisher
Watabe K, Niida Kawaguchi M, Tada M, Kato Y, Murata M, Tanji K, et al. Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation. Neuropathology. 2022;: pubmed publisher
Peinado J, Chaplot K, Jarvela T, Barbieri E, Shorter J, Lindberg I. Sequestration of TDP-43216-414 Aggregates by Cytoplasmic Expression of the proSAAS Chaperone. ACS Chem Neurosci. 2022;13:1651-1665 pubmed publisher
Palminha N, dos Santos Souza C, Griffin J, Liao C, Ferraiuolo L, El Khamisy S. Defective repair of topoisomerase I induced chromosomal damage in Huntington's disease. Cell Mol Life Sci. 2022;79:160 pubmed publisher
Qiu W, Ai W, Zhu F, Zhang Y, Guo M, Law B, et al. Polygala saponins inhibit NLRP3 inflammasome-mediated neuroinflammation via SHP-2-Mediated mitophagy. Free Radic Biol Med. 2021;179:76-94 pubmed publisher
Joshi B, Youssef S, Bron R, de Bruin A, Kampinga H, Zuhorn I. DNAJB6b-enriched small extracellular vesicles decrease polyglutamine aggregation in in vitro and in vivo models of Huntington disease. iScience. 2021;24:103282 pubmed publisher
Blumenstock S, Schulz Trieglaff E, Voelkl K, Bolender A, Lapios P, Lindner J, et al. Fluc-EGFP reporter mice reveal differential alterations of neuronal proteostasis in aging and disease. EMBO J. 2021;40:e107260 pubmed publisher
Zhang N, Bewick B, Schultz J, Tiwari A, Krencik R, Zhang A, et al. DNAzyme Cleavage of CAG Repeat RNA in Polyglutamine Diseases. Neurotherapeutics. 2021;: pubmed publisher
de Armas Rillo S, Fumagallo Reading F, Luis Ravelo D, Abdul Jalbar B, Gonzalez Hernandez T, Lahoz F. Random Lasing Detection of Mutant Huntingtin Expression in Cells. Sensors (Basel). 2021;21: pubmed publisher
Kim Guisbert K, Mossiah I, Guisbert E. Titration of SF3B1 Activity Reveals Distinct Effects on the Transcriptome and Cell Physiology. Int J Mol Sci. 2020;21: pubmed publisher
Aravindan S, Chen S, Choudhry H, Molfetta C, Chen K, Liu A. Osmolytes dynamically regulate mutant Huntingtin aggregation and CREB function in Huntington's disease cell models. Sci Rep. 2020;10:15511 pubmed publisher
Rodríguez González C, Lin S, Arkan S, Hansen C. Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation. Sci Rep. 2020;10:8130 pubmed publisher
Glaser T, Shimojo H, Ribeiro D, Martins P, Beco R, Kosinski M, et al. ATP and spontaneous calcium oscillations control neural stem cell fate determination in Huntington's disease: a novel approach for cell clock research. Mol Psychiatry. 2020;: pubmed publisher
Nath S, Lieberman M, Yu Z, Marchioretti C, Jones S, Danby E, et al. MEF2 impairment underlies skeletal muscle atrophy in polyglutamine disease. Acta Neuropathol. 2020;140:63-80 pubmed publisher
Nóbrega C, Conceição A, Costa R, Koppenol R, Sequeira R, Nunes R, et al. The cholesterol 24-hydroxylase activates autophagy and decreases mutant huntingtin build-up in a neuroblastoma culture model of Huntington's disease. BMC Res Notes. 2020;13:210 pubmed publisher
Pinho B, Reis S, Hartley R, Murphy M, Oliveira J. Mitochondrial superoxide generation induces a parkinsonian phenotype in zebrafish and huntingtin aggregation in human cells. Free Radic Biol Med. 2019;130:318-327 pubmed publisher
Chang C, Lin T, Ho H, Kuo C, Li H, Korolenko T, et al. GLP-1 Analogue Liraglutide Attenuates Mutant Huntingtin-Induced Neurotoxicity by Restoration of Neuronal Insulin Signaling. Int J Mol Sci. 2018;19: pubmed publisher
Chen J, Parekh M, Seliman H, Bakshinskaya D, Dai W, Kwan K, et al. Heat shock promotes inclusion body formation of mutant huntingtin (mHtt) and alleviates mHtt-induced transcription factor dysfunction. J Biol Chem. 2018;293:15581-15593 pubmed publisher
Jena K, Kolapalli S, Mehto S, Nath P, Das B, Sahoo P, et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. EMBO J. 2018;37: pubmed publisher
Zeng Y, Jones A, Thomas E, Nassif B, Silberg J, Segatori L. A Split Transcriptional Repressor That Links Protein Solubility to an Orthogonal Genetic Circuit. ACS Synth Biol. 2018;7:2126-2138 pubmed publisher
Pierzynowska K, Gaffke L, Hać A, Mantej J, Niedziałek N, Brokowska J, et al. Correction of Huntington's Disease Phenotype by Genistein-Induced Autophagy in the Cellular Model. Neuromolecular Med. 2018;20:112-123 pubmed publisher
Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
Tanji K, Mori F, Miki Y, Utsumi J, Sasaki H, Kakita A, et al. YOD1 attenuates neurogenic proteotoxicity through its deubiquitinating activity. Neurobiol Dis. 2018;112:14-23 pubmed publisher
Al Ramahi I, Giridharan S, Chen Y, Patnaik S, Safren N, Hasegawa J, et al. Inhibition of PIP4K? ameliorates the pathological effects of mutant huntingtin protein. elife. 2017;6: pubmed publisher
Clift D, McEwan W, Labzin L, Konieczny V, Mogessie B, James L, et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell. 2017;171:1692-1706.e18 pubmed publisher
Aprile F, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C. The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation. Sci Rep. 2017;7:9039 pubmed publisher
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
Barakat D, Mendonca J, Barberi T, Zhang J, Kachhap S, Paz Priel I, et al. C/EBPβ regulates sensitivity to bortezomib in prostate cancer cells by inducing REDD1 and autophagosome-lysosome fusion. Cancer Lett. 2016;375:152-161 pubmed publisher
Tanji K, Miki Y, Maruyama A, Mori F, Mimura J, Itoh K, et al. The role of NUB1 in α-synuclein degradation in Lewy body disease model mice. Biochem Biophys Res Commun. 2016;470:635-642 pubmed publisher
Yue F, Li W, Zou J, Chen Q, Xu G, Huang H, et al. Blocking the association of HDAC4 with MAP1S accelerates autophagy clearance of mutant Huntingtin. Aging (Albany NY). 2015;7:839-53 pubmed
Narain Y, Wyttenbach A, Rankin J, Furlong R, Rubinsztein D. A molecular investigation of true dominance in Huntington's disease. J Med Genet. 1999;36:739-46 pubmed
product information
Catalog Number :
40262
Product Name :
pEGFP-Q74
article :
doi10.1136/jmg.36.10.739
id5830
pubmed_id10528852
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
huntingtin
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BglII
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutationexpanded poly-Q
nameHTT partial exon 1 Q74
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
1372
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA