This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti4/V5-DEST-P85(R574fs)
catalog :
40227
citations: 1
Reference
Shi D, Savani M, Levitt M, Wang A, Endress J, Bird C, et al. De novo pyrimidine synthesis is a targetable vulnerability in IDH mutant glioma. Cancer Cell. 2022;40:939-956.e16 pubmed publisher
product information
Catalog Number :
40227
Product Name :
pLenti4/V5-DEST-P85(R574fs)
article :
doi
id5817
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepLenti4-V5/DEST
backbone_mutation
backbone_originInvitrogen
backbone_size6949
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
origin :
37
pi :
alt_names
PIK3R1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3V5 reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha
genePIK3R1
id5295
genbank_ids
mutationIntroduced frame shift mutation at amino acid 574
nameP85(R574fs)
shRNA_sequence
size2175
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
plasmid copy :
Flag-tagged human P85 construct was provided by Dr Jayne Stommel (National Cancer Institute).
resistance markers :
78
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA