This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pExodus CMV.Trex2
catalog :
40210
citations: 6
Reference
Kozhukhar N, Spadafora D, Rodriguez Y, Alexeyev M. A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication. Cells. 2022;11: pubmed publisher
Lainšček D, Forstnerič V, Mikolič V, Malenšek Š, Pečan P, Bencina M, et al. Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing. Nat Commun. 2022;13:3604 pubmed publisher
Renema P, Kozhukhar N, Pastukh V, Spadafora D, Subedi Paudel S, Tambe D, et al. Exoenzyme Y induces extracellular active caspase-7 accumulation independent from apoptosis: Modulation of transmissible cytotoxicity. Am J Physiol Lung Cell Mol Physiol. 2020;: pubmed publisher
Lee J, Alexeyev M, Kozhukhar N, Pastukh V, White R, Stevens T. Carbonic anhydrase IX is a critical determinant of pulmonary microvascular endothelial cell pH regulation and angiogenesis during acidosis. Am J Physiol Lung Cell Mol Physiol. 2018;315:L41-L51 pubmed publisher
Balczon R, Morrow K, Zhou C, Edmonds B, Alexeyev M, Pittet J, et al. Pseudomonas aeruginosa infection liberates transmissible, cytotoxic prion amyloids. FASEB J. 2017;31:2785-2796 pubmed publisher
Certo M, Gwiazda K, Kuhar R, Sather B, Curinga G, Mandt T, et al. Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Nat Methods. 2012;9:973-5 pubmed publisher
product information
Catalog Number :
40210
Product Name :
pExodus CMV.Trex2
article :
doi10.1038/nmeth.2177
id5980
pubmed_id22941364
bacterial resistance :
Ampicillin
cloning :
backbonepUC
backbone_mutationMultiple cloning site, CMV and T7 promoters
backbone_originIn-house
backbone_size4389
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Mammalian expression of codon-optimized Trex2.
origin :
37
pi :
alt_names
Trex2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5Nhe1
promoterCMV and T7
sequencing_primer_3BGH RP
sequencing_primer_5T7 FP
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneTrex2
id24102
genbank_ids
mutation
nameThree prime repair exonuclease 2
shRNA_sequence
size711
species
10090
Mus musculus
tags
plasmid copy :
We received the Trex2 ORF-containing construct from the laboratory of Daniel Stetson, University of Washington
resistance markers :
1000
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA