This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFM107
catalog :
40153
citations: 1
Reference
Motegi F, Zonies S, Hao Y, Cuenca A, Griffin E, Seydoux G. Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Nat Cell Biol. 2011;13:1361-7 pubmed publisher
product information
Catalog Number :
40153
Product Name :
pFM107
article :
doi10.1038/ncb2354
id5813
pubmed_id21983565
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6p-1
backbone_mutation
backbone_originGE
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Alternate plasmid name: pGEX6p1-par-2 7SA 7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A.
origin :
37
pi :
alt_names
par-2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promotertac
sequencing_primer_3pGEX3
sequencing_primer_5pGEX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_F58B6.3
genepar-2
id175275
genbank_ids
NC_003281.9
mutation7SA; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene.
namepar-2 RNAi resistant, 7SA
shRNA_sequence
size
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tagGST
resistance markers :
459
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA