pEG795 | Addgene 40122 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEG795

catalog :

40122

citations: 1

Reference
Griffin E, Odde D, Seydoux G. Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Cell. 2011;146:955-68 pubmed publisher

product information

Catalog Number :

40122

Product Name :

pEG795

article :

doi	10.1016/j.cell.2011.08.012
id	5812
pubmed_id	21925318

bacterial resistance :

Kanamycin

cloning :

backbone

pDONR P2RP3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

4773

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3 UTR

origin :

pi :

alt_names

Mex-5

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_W02A2.7
gene	mex-5
id	178296

genbank_ids

NM_070165.4

NP_502566.1

mutation

Exon 2 recoded to be RNAi-resistant

name

Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)

shRNA_sequence

size

species

6239

Caenorhabditis elegans