pEG345 | Addgene 40077 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEG345

catalog :

40077

citations: 2

Reference
Spike C, Tsukamoto T, Greenstein D. Ubiquitin ligases and a processive proteasome facilitate protein clearance during the oocyte-to-embryo transition in Caenorhabditis elegans. Genetics. 2022;221: pubmed publisher
Griffin E, Odde D, Seydoux G. Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Cell. 2011;146:955-68 pubmed publisher

product information

Catalog Number :

40077

Product Name :

pEG345

article :

doi	10.1016/j.cell.2011.08.012
id	5812
pubmed_id	21925318

bacterial resistance :

Ampicillin

cloning :

backbone

pIC26

backbone_mutation

GFP replaced with Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted)

backbone_origin

Cheeseman lab

backbone_size

16397

promoter

pie-1

sequencing_primer_3

sequencing_primer_5

vector_types

Worm Expression

growth notes :

MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004). Alternate plasmid name: pIC26 + MEX-5 (Dendra + MEX-5 ORF)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SpeI
cloning_site_5	SpeI
promoter	pie-1
sequencing_primer_3
sequencing_primer_5	DENDRA-F (GGGAACCATCGACTGAAAAA)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CELE_W02A2.7
gene	mex-5
id	178296

genbank_ids

NM_070165.4

NP_502566.1

mutation

name

Mex-5

shRNA_sequence

size

1407

species

6239

Caenorhabditis elegans