pRc/CMV HA TrkB-T1 | Addgene 39980 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRc/CMV HA TrkB-T1

catalog :

39980

citations: 1

Reference
Bibel M, Hoppe E, Barde Y. Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. EMBO J. 1999;18:616-22 pubmed

product information

Catalog Number :

39980

Product Name :

pRc/CMV HA TrkB-T1

article :

doi	10.1093/emboj/18.3.616
id	5774
pubmed_id	9927421

bacterial resistance :

Ampicillin

cloning :

backbone

pRC/CMV AC7 vector, a derivative of the pRC/CMV vector

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI. See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., https://www.ncbi.nlm.nih.gov/pubmed?term=1846020 ) to learn more about the TrkB.T1 variant. Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32

origin :

pi :

alt_names

NTrk2 variant 2

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RATTRKB1, TRKB1, Tkrb, trk-B, trkB
gene	Ntrk2
id	25054

genbank_ids

NM_001163168.1

mutation

TrkB.T1 variant; endogenous signal sequence was replaced with BM40 and HA tag

name

TrkB.T1

shRNA_sequence

size

1500

species

10116

Rattus norvegicus