This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRc/CMV HA TrkC
catalog :
39979
citations: 1
Reference
Bibel M, Hoppe E, Barde Y. Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. EMBO J. 1999;18:616-22 pubmed
product information
Catalog Number :
39979
Product Name :
pRc/CMV HA TrkC
article :
doi10.1093/emboj/18.3.616
id5774
pubmed_id9927421
bacterial resistance :
Ampicillin
cloning :
backbonepRC/CMV AC7 vector, a derivative of the pRC/CMV vector
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkC was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequence for GenBank ID NM_001270656.1, the TrkC protein sequence starts at amino acid #32
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI (PmeI added)
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasestrkC
geneNtrk3
id29613
genbank_ids
NM_001270656.1
mutationendogenous signal sequence was replaced with BM40 and HA tag
nameTrkC
shRNA_sequence
size2500
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagBM40 signal sequence
locationN terminal on insert
tagHA
resistance markers :
1337
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA