pRc/CMV HA TrkB | Addgene 39978 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRc/CMV HA TrkB

catalog :

39978

citations: 2

Reference
Burk K, Ramachandran B, Ahmed S, Hurtado Zavala J, Awasthi A, Benito E, et al. Regulation of Dendritic Spine Morphology in Hippocampal Neurons by Copine-6. Cereb Cortex. 2018;28:1087-1104 pubmed publisher
Bibel M, Hoppe E, Barde Y. Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. EMBO J. 1999;18:616-22 pubmed

product information

Catalog Number :

39978

Product Name :

pRc/CMV HA TrkB

article :

doi	10.1093/emboj/18.3.616
id	5774
pubmed_id	9927421

bacterial resistance :

Ampicillin

cloning :

backbone

pRC/CMV AC7 vector, a derivative of the pRC/CMV vector

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide Please note that the endogenous signal sequence of TrkB was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55291.1 or NM_012731.2, the TrkB protein sequence starts at amino acid #32

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	HindIII (PmeI before HindIII)
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RATTRKB1, TRKB1, Tkrb, trk-B, trkB
gene	Ntrk2
id	25054

genbank_ids

NM_012731.2

mutation

endogenous signal sequence was replaced with BM40 and HA tag

name

TrkB

shRNA_sequence

size

2500

species

10116

Rattus norvegicus