pcDNA3-cdc2-AF (718) | Addgene 39872 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3-cdc2-AF (718)

catalog :

39872

citations: 6

Reference
Cao X, Shami Shah A, Sanford E, Smolka M, Baskin J. Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor. ACS Chem Biol. 2022;17:2605-2618 pubmed publisher
Ponce R, Thomas N, Bui N, Kondo T, Okimoto R. WEE1 kinase is a therapeutic vulnerability in CIC-DUX4 undifferentiated sarcoma. JCI Insight. 2022;7: pubmed publisher
Yin L, Zeng Y, Zeng R, Chen Y, Wang T, Rodabaugh K, et al. Protein kinase RNA-activated controls mitotic progression and determines paclitaxel chemosensitivity through B-cell lymphoma 2 in ovarian cancer. Oncogene. 2021;40:6772-6785 pubmed publisher
Parekh U, McDonald D, Dailamy A, Wu Y, Cordes T, Zhang K, et al. Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas. iScience. 2021;24:103149 pubmed publisher
Zeng R, Wang Z, Li X, Chen Y, Yang S, Dong J. Cyclin-dependent kinase 1-mediated phosphorylation of protein kinase N1 promotes anchorage-independent growth and migration. Cell Signal. 2020;69:109546 pubmed publisher
Hagting A, Karlsson C, Clute P, Jackman M, Pines J. MPF localization is controlled by nuclear export. EMBO J. 1998;17:4127-38 pubmed

product information

Catalog Number :

39872

Product Name :

pcDNA3-cdc2-AF (718)

article :

doi	10.1093/emboj/17.14.4127
id	5795
pubmed_id	9670027

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Phosphorylation site mutant of cdc2/Cdk1 that cannot be down-regulated by Wee1. Please note that this construct may also have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDC2, CDC28A, P34CDC2
gene	CDK1
id	983

genbank_ids

mutation

AF = T14A, Y15F

name

Cdc2

shRNA_sequence

size

species

9606

Homo sapiens