This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CycA-Venus-Flag (1305)
catalog :
39853
citations: 2
Reference
Rouland L, Duplan E, Ramos Dos Santos L, Bernardin A, Katula K, Manfioletti G, et al. Therapeutic potential of parkin as a tumor suppressor via transcriptional control of cyclins in glioblastoma cell and animal models. Theranostics. 2021;11:10047-10063 pubmed publisher
Di Fiore B, Pines J. How cyclin A destruction escapes the spindle assembly checkpoint. J Cell Biol. 2010;190:501-9 pubmed publisher
product information
Catalog Number :
39853
Product Name :
CycA-Venus-Flag (1305)
article :
doi10.1083/jcb.201001083
id5764
pubmed_id20733051
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5/FRT/TO
backbone_mutationFirst, Venus-Flag was added to pcDNA5/FRT/TO between BstXI and XhoI. Second, an attR1-CmR-ccdB-attR2 GATEWAY cassette was inserted into at the AflII site.
backbone_originPines lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3BGH_rev_primer
sequencing_primer_5CMV fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCCN1, CCNA
geneCCNA2
id890
genbank_ids
mutation
nameCyclin A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagVenus
locationC terminal on backbone
tagFlag
resistance markers :
790
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA