This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pVenus_Lys-less-Cdc20 (1620)
catalog :
39849
citations: 1
Reference
Nilsson J, Yekezare M, Minshull J, Pines J. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nat Cell Biol. 2008;10:1411-20 pubmed publisher
product information
Catalog Number :
39849
Product Name :
pVenus_Lys-less-Cdc20 (1620)
article :
doi10.1038/ncb1799
id3552
pubmed_id18997788
bacterial resistance :
Kanamycin
cloning :
backbonepVenus-C1
backbone_mutationEYFP in pEYFP-C1 was swapped for Venus using AgeI and BsrGI.
backbone_originPines lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
RNAi resistant Cdc20 that cannot be ubiquitinated.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC
geneCDC20
id991
genbank_ids
mutationLys-less = all Lys converted to Arg
nameCdc20
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagVenus
resistance markers :
790
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA