This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-puro
catalog :
39481
citations: 70
Reference
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product information
Catalog Number :
39481
Product Name :
pLenti-puro
article :
doi10.1158/0008-5472.CAN-11-1562
id5711
pubmed_id21900401
bacterial resistance :
Ampicillin
cloning :
backbonepLenti4/TO/V5-DEST
backbone_mutationRemoved the Gateway elements between the 2 EcoRV sites and replaced the Zeocin resistance gene with a puromycin resistance gene
backbone_originInvitrogen
backbone_size
promoterCMV
sequencing_primer_3V5-rev and BGH-Rev
sequencing_primer_5CMV-fwd
vector_types
Mammalian Expression
Lentiviral
growth notes :
Please note this plasmid appears to be unstable during growth in bacteria. We recommend screening 3-5 smaller colonies after streaking from the original stab. It may be necessary to cut the plasmid with a single-cutting enzyme, gel purify the high molecular weight band, and religate to restore the plasmid.
growth strain :
Tetracycline inducible 3rd generation lentiviral vector
growth temp :
See Depositor Comments below.
origin :
37
resistance markers :
1331
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA