This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
anti-Notch1_E6-pBIOCAM5
catalog :
39344
citations: 6
Reference
Sloas D, Tran J, Marzilli A, Ngo J. Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Nat Biotechnol. 2023;: pubmed publisher
Rabia L, Zhang Y, Ludwig S, Julian M, Tessier P. Net charge of antibody complementarity-determining regions is a key predictor of specificity. Protein Eng Des Sel. 2018;31:409-418 pubmed publisher
Zheng L, Conner S. PI5P4K? functions in DTX1-mediated Notch signaling. Proc Natl Acad Sci U S A. 2018;115:E1983-E1990 pubmed publisher
Zheng L, Conner S. Glycogen synthase kinase 3β inhibition enhances Notch1 recycling. Mol Biol Cell. 2018;29:389-395 pubmed publisher
Dyson M. Fundamentals of Expression in Mammalian Cells. Adv Exp Med Biol. 2016;896:217-24 pubmed publisher
Falk R, Falk A, Dyson M, Melidoni A, Parthiban K, Young J, et al. Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods. 2012;58:69-78 pubmed publisher
product information
Catalog Number :
39344
Product Name :
anti-Notch1_E6-pBIOCAM5
article :
doi10.1016/j.ymeth.2012.07.008
id7083
pubmed_id22842086
bacterial resistance :
Ampicillin
cloning :
backbonepBIOCAM5-3F
backbone_mutation
backbone_originMcCafferty lab unpublished
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Binds to both mouse mNRR1 and human hNRR1 but not NRR2.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NcoI
promoterCMV
sequencing_primer_3BGH_rev_primer
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases9930111A19Rik, Mis6, N1, Tan1, lin-12
geneNotch1
id18128
aliasesAOS5, AOVD1, TAN1, hN1
geneNOTCH1
id4851
genbank_ids
mutation
namescFv-Fc-fusion specific for Notch1 (clone B9)
shRNA_sequence
size
species
9606
Homo sapiens
10090
Mus musculus
tags
locationC terminal on backbone
taghuman Fc
locationC terminal on backbone
tag6xHis
locationC terminal on backbone
tag3xFLAG
resistance markers :
1326
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA