This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFA6a-kanMX6-P41nmt1-GFP
catalog :
39290
citations: 2
Reference
Andric V, Nevers A, Hazra D, Auxilien S, Menant A, Graille M, et al. A scaffold lncRNA shapes the mitosis to meiosis switch. Nat Commun. 2021;12:770 pubmed publisher
Bahler J, Wu J, Longtine M, Shah N, McKenzie A, Steever A, et al. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast. 1998;14:943-51 pubmed
product information
Catalog Number :
39290
Product Name :
pFA6a-kanMX6-P41nmt1-GFP
article :
doi10.1002/(SICI)1097-0061(199807)14:10<943::AID-YEA292>3.0.CO;2-Y
id5710
pubmed_id9717240
bacterial resistance :
Ampicillin
cloning :
backbonepFA6a
backbone_mutation
backbone_origin
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Yeast genomic targeting
growth notes :
Use GFP reverse primer to verify mutation in nmt1 promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PacI
cloning_site_5BglII
promoter
sequencing_primer_3T7_promoter
sequencing_primer_5Sp6_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPCC1223.02
genenmt1
id2539108
genbank_ids
mutation-1163 to +6 of nmt1 locus with a 4bp deletion (TATA) ~100bp from 3' end of promoter
namenmt1 promoter
shRNA_sequence
size2900
species
4896
Schizosaccharomyces pombe
tags
locationN terminal on insert
tagA. gossypii translation elongation factor 1a gene terminator
locationN terminal on insert
tagKanR reverse orientation
locationN terminal on insert
tagA. gossypii translation elongation factor 1a gene promoter
locationC terminal on insert
tagGFP
locationC terminal on insert
tagTADH1
resistance markers :
1325
1324
tags :
Unknown
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA