This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-His6-ERK2-MEK1_R4F_coexpression
catalog :
39212
citations: 10
Reference
Solone X, Caldara A, Wells B, Qiao H, Wade L, Salerno J, et al. MAP kinases differentially bind and phosphorylate NOS3 via two unique NOS3 sites. FEBS Open Bio. 2022;12:1075-1086 pubmed publisher
Lai K, Galan S, Zeng Y, Zhou T, He C, Raj R, et al. LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome. Cell. 2021;184:2680-2695.e26 pubmed publisher
Tavernier N, Thomas Y, Vigneron S, Maisonneuve P, Orlicky S, Mader P, et al. Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nat Commun. 2021;12:1899 pubmed publisher
Wigington C, Roy J, Damle N, Yadav V, Blikstad C, Resch E, et al. Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling. Mol Cell. 2020;79:342-358.e12 pubmed publisher
Mayfield J, Irani S, Escobar E, Zhang Z, Burkholder N, Robinson M, et al. Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb. elife. 2019;8: pubmed publisher
Meksiriporn B, Ludwicki M, Stephens E, Jiang A, Lee H, Waraho Zhmayev D, et al. A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins. Nat Commun. 2019;10:1830 pubmed publisher
Irani S, Sipe S, Yang W, Burkholder N, Lin B, Sim K, et al. Structural determinants for accurate dephosphorylation of RNA polymerase II by its cognate C-terminal domain (CTD) phosphatase during eukaryotic transcription. J Biol Chem. 2019;294:8592-8605 pubmed publisher
Chatzifrangkeskou M, Yadin D, Marais T, Chardonnet S, Cohen Tannoudji M, Mougenot N, et al. Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated cardiomyopathy caused by lamin A/C gene mutation. Hum Mol Genet. 2018;27:3060-3078 pubmed publisher
Weijman J, Riedl S, Mace P. Structural Studies of ERK2 Protein Complexes. Methods Mol Biol. 2017;1487:53-63 pubmed
Khokhlatchev A, Xu S, English J, Wu P, Schaefer E, Cobb M. Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases. J Biol Chem. 1997;272:11057-62 pubmed
product information
Catalog Number :
39212
Product Name :
pET-His6-ERK2-MEK1_R4F_coexpression
article :
doi10.1074/jbc.272.17.11057
id5675
pubmed_id9110999
bacterial resistance :
Ampicillin
cloning :
backbonepET
backbone_mutation
backbone_origin
backbone_size4600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NA
cloning_site_5NA
promoterT7
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
mutationMEK1(R4F) = S218E, S222D and deletion of AA32-51
nameMek1 R4F
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagRBS
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NA
cloning_site_5NA
promoterT7 via RBS
sequencing_primer_3rErk2-R
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERK-2, ERT1, Erk2, p42-MAPK
geneMapk1
id116590
genbank_ids
mutation
nameErk2
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagHis6
resistance markers :
83
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA