This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YCplac111-KOG1 (pAN58)
catalog :
39209
citations: 1
| Reference |
|---|
Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo J, Bonenfant D, et al. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell. 2002;10:457-68 pubmed
|
product information
Catalog Number :
39209
Product Name :
YCplac111-KOG1 (pAN58)
article :
| doi | 10.1016/S1097-2765(02)00636-6 |
| id | 5681 |
| pubmed_id | 12408816 |
bacterial resistance :
Ampicillin
cloning :
| backbone | YCplac111 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 6100 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Expresses 2x-myc-tagged KOG1 under control of KOG1 promoter. The two myc-tags are integrated between codons 1 and 2 of KOG1. A 6.0 kb PCR fragment containing the KOG1 locus ( 972 to +304 with respect to the ORF) was cloned into the XbaI and Asp718I sites of YCplac111; the sequence ATG ATC CCC GGG TTA ATT AAC GGT GAA CAA AAG CTA ATC TCC GAG GAA GAC TTG AAC GGT GAA CAA AAA TTA ATC TCA GAA GAA GAC TTG AAC GGA CTC GAC GGT GAA CAA encoding two myc tags was integrated between codons 1 and 2 of KOG1 Addgene's sequencing result identified a L1512P mutation in the KOG1 sequence when compared to GenBank reference sequence NP_012056.1. The depositing laboratory confirmed the plasmid is functional as described in the associated publication.
origin :
37
pi :
|
resistance markers :
| 1316 |
tags :
Low Copy
terms :
| LEU2 |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments