This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-myc-ERK2-L4A-MEK1_fusion
catalog :
39197
citations: 5
Reference
Park J, Park G, Lee J, Park J, Kim S, Kim E, et al. ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growth. Front Mol Biosci. 2022;9:1030725 pubmed publisher
Liu J, Hideshima T, Xing L, Wang S, Zhou W, Samur M, et al. ERK signaling mediates resistance to immunomodulatory drugs in the bone marrow microenvironment. Sci Adv. 2021;7: pubmed publisher
Mereness J, Bhattacharya S, Wang Q, Ren Y, Pryhuber G, Mariani T. Type VI collagen promotes lung epithelial cell spreading and wound-closure. PLoS ONE. 2018;13:e0209095 pubmed publisher
Li C, Götz J. Somatodendritic accumulation of Tau in Alzheimer's disease is promoted by Fyn-mediated local protein translation. EMBO J. 2017;36:3120-3138 pubmed publisher
Caropreso V, Darvishi E, Turbyville T, Ratnayake R, Grohar P, McMahon J, et al. Englerin A Inhibits EWS-FLI1 DNA Binding in Ewing Sarcoma Cells. J Biol Chem. 2016;291:10058-66 pubmed publisher
product information
Catalog Number :
39197
Product Name :
pCMV-myc-ERK2-L4A-MEK1_fusion
article :
doi10.1016/S0960-9822(07)00485-X
id5673
pubmed_id9799732
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
rat ERK2fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES
inserts :
TG1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5KpnI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
aliasesERK-2, ERT1, Erk2, p42-MAPK
geneMapk1
id116590
genbank_ids
M64300
NM_002755
mutationL4A = L33A, L37A, L40A, L42A
nameERK2-MEK1 Fusion
shRNA_sequence
size2400
species
9606
Homo sapiens
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagMyc
resistance markers :
83
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA