phOct4-EGFP | Addgene 38776 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

phOct4-EGFP

catalog :

38776

citations: 5

Reference
Tan A, PRASAD R, Jho E. TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis. Cell Death Dis. 2021;12:343 pubmed publisher
Patel A, Vipparthi K, Thatikonda V, Arun I, Bhattacharjee S, Sharan R, et al. A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma. Oncogenesis. 2018;7:78 pubmed publisher
Abel E, Goto M, Magnuson B, Abraham S, Ramanathan N, Hotaling E, et al. HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties. elife. 2018;7: pubmed publisher
Vega Crespo A, Truong B, Hermann K, Awe J, Chang K, Lee P, et al. Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells. Mol Ther Methods Clin Dev. 2016;3:16050 pubmed publisher
Gerrard L, Zhao D, Clark A, Cui W. Stably transfected human embryonic stem cell clones express OCT4-specific green fluorescent protein and maintain self-renewal and pluripotency. Stem Cells. 2005;23:124-33 pubmed

product information

Catalog Number :

38776

Product Name :

phOct4-EGFP

article :

doi	10.1634/stemcells.2004-0102
id	5667
pubmed_id	15625129

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-1

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The human OCT4 promoter (hOCT4pr, from 67539 to 71490 in human DNA sequence with accession number AP000509) was amplified by PCR with primers: hOCT4pr-F (5' TTCCCATGTCAAGTAAGTGGGGTGG-3') and hOCT4pr-R (5'-CGAGAAGGCAAAATCTGAAGCCAGG-3') using human genomic DNA (Promega G3041) as a template. The fragment was cloned into a TOPO vector and the hOCT4 promoter was subsequently cloned into vector pEGFP1 by insertion into HindIII and AgeI restriction enzyme sites upstream of EGFP.

origin :

pi :

alt_names

cloning

clone_method	TOPO Cloning
cloning_site_3
cloning_site_5
promoter	human OCT4
sequencing_primer_3	CGA GAA GGC AAA ATC TGA AGC CAG G
sequencing_primer_5	TT CCC ATG TCA AGT AAG TGG GGT GG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

mutation

name

Oct-4 promoter

shRNA_sequence

size

3950

species

9606

Homo sapiens