This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCM-HA-Gas1
catalog :
38313
citations: 1
Reference
McLellan C, Whitesell L, King O, Lancaster A, Mazitschek R, Lindquist S. Inhibiting GPI anchor biosynthesis in fungi stresses the endoplasmic reticulum and enhances immunogenicity. ACS Chem Biol. 2012;7:1520-8 pubmed
product information
Catalog Number :
38313
Product Name :
pCM-HA-Gas1
article :
doi10.1021/cb300235m
id5639
pubmed_id22724584
bacterial resistance :
Ampicillin
cloning :
backbonepAG416Gal-ccdB
backbone_mutationThe gal promoter was removed from pAG416GalccdB by cutting with SacI (2792) and XbaI (3255, making blunt ends which were filled with Klenow and then ligated.
backbone_originAddgene 14147
backbone_size5173
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
The primers for the genomic PCR and the location of the HA tag are from Fujita, MBC, 17,834,2006.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEndogenous
sequencing_primer_3M13F
sequencing_primer_5M13R
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYMR307W, CWH52, GGP1
geneGAS1
id855355
genbank_ids
mutationcontains promoter region
nameGas1
shRNA_sequence
size2938
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tag2xHA
resistance markers :
293
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA