This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMXs-IP spGFP-ERGIC53
catalog :
38270
citations: 3
Reference
Wilson Z, Jiang M, Geng J, Kaur S, Workman S, Hao J, et al. Delayed KCNQ1/KCNE1 assembly on the cell surface helps IKs fulfil its function as a repolarization reserve in the heart. J Physiol. 2021;599:3337-3361 pubmed publisher
Bowen A, Bourke A, Hiester B, Hanus C, Kennedy M. Golgi-independent secretory trafficking through recycling endosomes in neuronal dendrites and spines. elife. 2017;6: pubmed publisher
Itakura E, Mizushima N. Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy. 2010;6:764-76 pubmed
product information
Catalog Number :
38270
Product Name :
pMXs-IP spGFP-ERGIC53
article :
doi10.4161/auto.6.6.12709
id5631
pubmed_id20639694
bacterial resistance :
Ampicillin
cloning :
backbonepMXs-IP
backbone_mutation
backbone_originDr. Toshio Kitamura of the University of Tokyo
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
origin :
37
pi :
alt_names
ER-Golgi intermediate compartment 53 kDa protein
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoter
sequencing_primer_3GAGGAACTGCTTCCTTCACG
sequencing_primer_5CGACCACTACCAGCAGAACA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58
geneLMAN1
id3998
genbank_ids
U09716
mutationdeleted signal peptide (1-30 amino acids)
nameERGIC-53
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP with Calreticulin signal peptide at N-terminal
plasmid copy :
ORFeome Collaboration clone, on13634
resistance markers :
644
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA