BCR/ABL P190 transgenic construct | Addgene 38185 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

BCR/ABL P190 transgenic construct

catalog :

38185

citations: 4

Reference
Witkowski M, Lee S, Wang E, Lee A, Talbot A, Ma C, et al. NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia. Nat Immunol. 2022;23:1424-1432 pubmed publisher
Ma C, Witkowski M, Harris J, Dolgalev I, Sreeram S, Qian W, et al. Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche. Sci Adv. 2020;6: pubmed publisher
Witkowski M, Dolgalev I, Evensen N, Ma C, Chambers T, Roberts K, et al. Extensive Remodeling of the Immune Microenvironment in B Cell Acute Lymphoblastic Leukemia. Cancer Cell. 2020;37:867-882.e12 pubmed publisher
Heisterkamp N, Jenster G, ten Hoeve J, Zovich D, Pattengale P, Groffen J. Acute leukaemia in bcr/abl transgenic mice. Nature. 1990;344:251-3 pubmed

product information

Catalog Number :

38185

Product Name :

BCR/ABL P190 transgenic construct

article :

doi	10.1038/344251a0
id	5622
pubmed_id	2179728

bacterial resistance :

Ampicillin

cloning :

backbone

pSK

backbone_mutation

backbone_origin

backbone_size

3000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

construct for making transgenic mice

growth notes :

The DNA construct was made by Guido Jenster and Nora Heisterkamp in several cloning and subcloning steps. The transgenic construct contains human BCR exon 1, intron sequences of human BCR and ABL genes, human ABL exon 2 and ABL cDNA sequences. The transgene expression is controlled by a 200-bp Sst I- Bgl II fragment of the murine metallothionein-1 (Mt1) promoter lacking glucocorticoid-responsive elements (PMID 8541551). This BCR/ABL fusion gene produces a P190 protein and is a model for Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL) in humans, which is caused by a chromosomal translocation that fuses the ABL proto-oncogene with breakpoint cluster region (BCR) sequences. Leukemia development is relatively rapid on inbred C57Bl/6J and CBA backgrounds and is slower on mixed genetic backgrounds. Mice transgenic for this construct on an inbred C57Bl/6J background are also available from Jackson labs stock 017833. The insert can be separated from the vector by digestion with Sst II.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Sst II
cloning_site_5	Sst II
promoter	truncated mouse MT promoter
sequencing_primer_3	M13-rev(?)
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
gene	ABL1
id	25

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

name

BCR/ABL P190

shRNA_sequence

size

10000

species

9606

Homo sapiens