Bcr DH domain NE/AA pGEX | Addgene 38180 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr DH domain NE/AA pGEX

catalog :

38180

citations: 1

Reference
Cho Y, Cunnick J, Yi S, Kaartinen V, Groffen J, Heisterkamp N. Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Mol Cell Biol. 2007;27:899-911 pubmed

product information

Catalog Number :

38180

Product Name :

Bcr DH domain NE/AA pGEX

article :

doi	10.1128/MCB.00756-06
id	4686
pubmed_id	17116687

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX 3X

backbone_mutation

backbone_origin

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Constructed by Young Jin Cho. Mutagenesis done using a Stratagene Quick Change kit. The parent plasmid Bcr DH domain in pGEX 3X (AddGene #38179) was digested with SstIxPshAI and a 430 bp fragment containing the wt sequence was removed. This fragment was replaced by a fragment containing the NE/AA mutations from ECFP-Bcr N689A/E690A (AddGene #36418). The insert includes nucleotides 2118-2965 of BCR NM_004327.

inserts :

Rosetta

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pGEX3
sequencing_primer_5	GST-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

NM_004327

mutation

BCR DH domain N689A/E690A

name

BCR DH domain N689A/E690A

shRNA_sequence

size

850

species

9606

Homo sapiens