pCDNA3.1Xpress-BcrGAP R1090A | Addgene 38176 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA3.1Xpress-BcrGAP R1090A

catalog :

38176

citations: 1

Reference
Cho Y, Cunnick J, Yi S, Kaartinen V, Groffen J, Heisterkamp N. Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Mol Cell Biol. 2007;27:899-911 pubmed

product information

Catalog Number :

38176

Product Name :

pCDNA3.1Xpress-BcrGAP R1090A

article :

doi	10.1128/MCB.00756-06
id	4686
pubmed_id	17116687

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Constructed by Young Jin Cho. pcDNA3.1Xpress-BcrGAP plasmid(AddGene #38175) was digested by BstEII x EcoRI and a 1.2 kb fragment containing the R1090 wt site, TAG and 3 UT sequences removed. The wt fragment was replaced by a 0.6 kb BstEII x EcoRI fragment containing the R1090A mutation isolated from EGFP-BCR R1090A pEGFP (AddGene #38173). The construct contains the natural TAG and 3 UT sequences. The difference between wt and mutant plasmids is in the 3 UT region. The construct includes amino acid residues 1004-1271.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Eco RI
cloning_site_5	Bam HI
promoter
sequencing_primer_3	BGH-Rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

aa 1004-1271 with R1090A point mutation

name

BCR GAP domain encoding residues 1004-1271 with R1090A point mutation

shRNA_sequence

size

800

species

9606

Homo sapiens