This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GST-Bcr pLEF
catalog :
38160
citations: 1
Reference
Mishra S, Reichert A, Cunnick J, Senadheera D, Hemmeryckx B, Heisterkamp N, et al. Protein kinase CKIIalpha interacts with the Bcr moiety of Bcr/Abl and mediates proliferation of Bcr/Abl-expressing cells. Oncogene. 2003;22:8255-62 pubmed
product information
Catalog Number :
38160
Product Name :
GST-Bcr pLEF
article :
doi10.1038/sj.onc.1207156
id5617
pubmed_id14614449
bacterial resistance :
Ampicillin
cloning :
backbonepLEF
backbone_mutation
backbone_origin
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Constructed by Anja Reichert. Generated in a 3-way ligation consisting of 5' 0.6 kb BamHI/SalI + 3' 3.5 kb SalI/XbaI BCR fragments into pLEF digested with BamHIxXbaI. The BCR insert contains the natural TAG and 3' UT sequences. At the 3' end there are polylinker sequences from pSK.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba I
cloning_site_5BamHI
promoterSV40
sequencing_primer_3SP6
sequencing_primer_5T7 or GST
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALL, BCR1, CML, D22S11, D22S662, PHL
geneBCR
id613
genbank_ids
mutation
nameBcr
shRNA_sequence
size4300
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST
resistance markers :
995
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA