Bcr/Abl P190-pLEF | Addgene 38159 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr/Abl P190-pLEF

catalog :

38159

citations: 1

Reference
Kweon S, Cho Y, Minoo P, Groffen J, Heisterkamp N. Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor. J Biol Chem. 2008;283:3023-30 pubmed

product information

Catalog Number :

38159

Product Name :

Bcr/Abl P190-pLEF

article :

doi	10.1074/jbc.M705513200
id	5616
pubmed_id	18070886

bacterial resistance :

Ampicillin

cloning :

backbone

pLEF

backbone_mutation

backbone_origin

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 6.3 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences. Addgene NGS identified a sequence discrepancy at nucleotide 3168 resulting in a T117M mutation in the ABL-1 sequence, relative to Genbank ID NP_005148.2 and Addgene Plasmid #38158. This is not a known polymorphism and the effect of this, if any, on the protein is not known.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	SV40
sequencing_primer_3	SP6
sequencing_primer_5	GST or T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
gene	ABL1
id	25

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

T117M in c-ABL1

name

BCR/ABL P190

shRNA_sequence

size

7000

species

9606

Homo sapiens