Bcr/Abl P210-pLEF | Addgene 38158 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Bcr/Abl P210-pLEF

catalog :

38158

citations: 5

Reference
Mendivil Perez M, Jimenez Del Rio M, Velez Pardo C. TPEN/TPGS (T2) combo dramatically reduces Philadelphia chromosome-positive pro-lymphoblastic B leukemia in BALB/c mice. Med Oncol. 2022;40:15 pubmed publisher
Huang X, Li Y, Shou L, Li L, Chen Z, Ye X, et al. The molecular mechanisms underlying BCR/ABL degradation in chronic myeloid leukemia cells promoted by Beclin1-mediated autophagy. Cancer Manag Res. 2019;11:5197-5208 pubmed publisher
Gudernová I, Foldynova Trantirkova S, Ghannamova B, Fafilek B, Varecha M, Balek L, et al. One reporter for in-cell activity profiling of majority of protein kinase oncogenes. elife. 2017;6: pubmed publisher
Colecchia D, Rossi M, Sasdelli F, Sanzone S, Strambi A, Chiariello M. MAPK15 mediates BCR-ABL1-induced autophagy and regulates oncogene-dependent cell proliferation and tumor formation. Autophagy. 2015;11:1790-802 pubmed publisher
Kweon S, Cho Y, Minoo P, Groffen J, Heisterkamp N. Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor. J Biol Chem. 2008;283:3023-30 pubmed

product information

Catalog Number :

38158

Product Name :

Bcr/Abl P210-pLEF

article :

doi	10.1074/jbc.M705513200
id	5616
pubmed_id	18070886

bacterial resistance :

Ampicillin

cloning :

backbone

pLEF

backbone_mutation

backbone_origin

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 7.7 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	SV40
sequencing_primer_3	SP6
sequencing_primer_5	T7 or GST
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl
gene	ABL1
id	25

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

name

BCR/ABL P210

shRNA_sequence

size

8450

species

9606

Homo sapiens