ECFP-Bcr in pCCL-cppt178-MNDU3 | Addgene 38156 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

ECFP-Bcr in pCCL-cppt178-MNDU3

catalog :

38156

citations: 1

Reference
Cho Y, Cunnick J, Yi S, Kaartinen V, Groffen J, Heisterkamp N. Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Mol Cell Biol. 2007;27:899-911 pubmed

product information

Catalog Number :

38156

Product Name :

ECFP-Bcr in pCCL-cppt178-MNDU3

article :

doi	10.1128/MCB.00756-06
id	4686
pubmed_id	17116687

bacterial resistance :

Ampicillin

cloning :

backbone

pCCL-cppt178-MNDU3

backbone_mutation

backbone_origin

Don Kohn dkohn@mednet.ucla.edu

backbone_size

6600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Constructed by Jess Cunnick. The construct was made in two steps. First a 5' 1.7 kb BamHIxSstI fragment and a 3' SstIxKpnI fragment from two BCR plasmids were ligated into pECFP-C1 digested with Bgl II x KpnI. ECFP-Bcr was removed from the plasmid by digestion with Age I x KpnI and ligated into pCCL0cppt178-MNDU3-X3.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Kpn I
cloning_site_5	Age I
promoter
sequencing_primer_3
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	ALL, BCR1, CML, D22S11, D22S662, PHL
gene	BCR
id	613

genbank_ids

mutation

name

Bcr

shRNA_sequence

size

4300

species

9606

Homo sapiens