N-cadherin in pCCL-c-MNDU3c-PGK-EGFP | Addgene 38153 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

catalog :

38153

citations: 5

Reference
Seo T, Lowery A, Xu H, Giang W, Troyanovsky S, Vincent P, et al. MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins. bioRxiv. 2023;: pubmed publisher
Quintanal Villalonga Á, Ferrer I, Guruceaga E, Cirauqui C, Marrugal A, Ojeda L, et al. FGFR1 and FGFR4 oncogenicity depends on n-cadherin and their co-expression may predict FGFR-targeted therapy efficacy. EBioMedicine. 2020;53:102683 pubmed publisher
Bagati A, Bianchi Smiraglia A, Moparthy S, Kolesnikova K, Fink E, Lipchick B, et al. Melanoma Suppressor Functions of the Carcinoma Oncogene FOXQ1. Cell Rep. 2017;20:2820-2832 pubmed publisher
Hsu C, Huang S, Wang J, Chu W, Nien J, Chen W, et al. Interplay of N-Cadherin and matrix metalloproteinase 9 enhances human nasopharyngeal carcinoma cell invasion. BMC Cancer. 2016;16:800 pubmed
Zhang B, Groffen J, Heisterkamp N. Increased resistance to a farnesyltransferase inhibitor by N-cadherin expression in Bcr/Abl-P190 lymphoblastic leukemia cells. Leukemia. 2007;21:1189-97 pubmed

product information

Catalog Number :

38153

Product Name :

N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

article :

doi	10.1038/sj.leu.2404667
id	5615
pubmed_id	17392819

bacterial resistance :

Ampicillin

cloning :

backbone

pCCL-c-MNDU3c-(X2)-Pgk-EGFP

backbone_mutation

backbone_origin

Don Kohn dkohn@mednet.ucla.edu

backbone_size

7900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Constructed by Bin Zhang. Vector was digested with EcoRI and the site blunted by filling in with T4 DNA polymerase. N-cadherin was isolated as a blunt-end fragment by digesting N-cadherin in pCMV6-XL with BsrBI (in front of the ATG) and with SmaI (in the vector). Has good N-cadherin expression. This vector expresses EGFP from the PGK promoter and non-tagged human N-cadherin.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter	MNDU3c = LTR promoter for U3 region of myeloproliferative sarcoma retrovirus MND
sequencing_primer_3	EGFP-N
sequencing_primer_5	MSCV_rev
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	ACOGS, ADHD8, ARVD14, CD325, CDHN, CDw325, NCAD
gene	CDH2
id	1000

genbank_ids

NM_001792

mutation

name

N-cadherin

shRNA_sequence

size

4100

species

9606

Homo sapiens